Transcriptome signature of mouse embryonic stem cell-derived microglia is closely related to primary cultured microglia

ABSTRACT: Preparation of primary microglial cultures from postnatal mice is tedious with a low yield, high variability and risk of astrocytic contamination. Microglia derived from embryonic stem cells (ESdM) have been suggested as alternative source, but it is unclear how closely ESdM resemble the molecular phenotype of primary microglia. Here, we performed a whole transcriptome analysis of ESdM in comparison to primary cultured and flow cytometry-sorted microglia and compared the microglial transcriptome to other cell types. Cultured microglia and ESdM were related to sorted microglia, but clearly distinct from other myeloid cell types, T cells, astrocytes and neurons. ESdM and primary cultured microglia showed strong overlap in their transcriptome. Only 144 gene transcripts were differentially exp

ORGANISM(S): Mus musculus

SUBMITTER: Joachim Schultze 

PROVIDER: E-GEOD-43808 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress