Systematic discovery of potent cell differentiation-directing transcription factors

ABSTRACT: A network of transcription factors (TFs) determines cell identity, but identity can be altered by overexpressing a combination of TFs. In principle, this opens the possibility of achieving one of the goals of regenerative medicine generating the desired differentiated cells from pluripotent stem cells, such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. However, choosing and verifying combinations of TFs for specific cell differentiation has been daunting due to a large number of possible combinations of ~2,000 TFs. Here we report an unbiased systematic approach identifying individual TFs that can direct efficient and rapid specific lineage differentiation. We start from a correlation matrix of global gene expression responses to the induction of single TFs and glob

ORGANISM(S): Mus musculus

SUBMITTER: Minoru Ko 

PROVIDER: E-GEOD-43971 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress