A global toxicogenomic analysis investigating the mechanistic differences between tobacco and marijuana smoke condensates in vitro

ABSTRACT: Like tobacco smoking, habitual marijuana smoking causes numerous adverse pulmonary effects. However, the mechanisms of action involved, especially as compared to tobacco smoke, are still unclear. To uncover putative modes of action, this study employed a toxicogenomics approach to compare the toxicological pathways perturbed following exposure to marijuana and tobacco smoke condensate in vitro. Condensates of mainstream smoke from hand-rolled tobacco and marijuana cigarettes were similarly prepared using identical smoking conditions. Murine lung epithelial cells were exposed to low, medium and high concentrations of the smoke condensates for 6 hr. RNA was extracted immediately or after a 4-hr recovery period and hybridized to mouse whole genome microarrays. Tobacco smoke condensate (TSC) e

ORGANISM(S): Mus musculus

SUBMITTER: Andrew Williams 

PROVIDER: E-GEOD-44603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress