Project description:Analysis of DOCK8 deficient animals revealed a novel marker of NKT cell development, the integrin CD103. The role of CD103 was further investigated by RNA microarray comparing CD103 negative versus positive NKT cells. Total RNA was extracted from 3 biological replicates of thymic, CD103positive NKT cells and compared using an Illumina microarray to CD103negative NKT cells.
Project description:Analysis of DOCK8 deficient animals revealed a key role for this protein the survival and maintenance of natural killer T cells. This work lead to the identification of genes regulated by the guanine exchange factor, DOCK8. Total RNA was extracted from 3 biological replicates of thymic, DOCK8 deficient NKT cells and compared using an Illumina microarray to WT NKT cells.
Project description:Analysis of DOCK8 deficient animals revealed a novel marker of NKT cell development, the integrin CD103. The role of CD103 was further investigated by RNA microarray comparing CD103 negative versus positive NKT cells.
Project description:Analysis of DOCK8 deficient animals revealed a key role for this protein the survival and maintenance of natural killer T cells. This work lead to the identification of genes regulated by the guanine exchange factor, DOCK8.
Project description:This study was designed to assess the global transcriptional differences between villin-Grem villi compared to normal villi of age-matched mice. We found changes in key gene signatures asscoiated with tumorigenesis. Total RNA obtained from isolated villi from Villin-Gremlin mouse (n=6) and wildtype controls (n=6)
Project description:Genome-wide methylation patterns observed in primary human leukocyte subsets were used to identify cell-typeM-^Vspecific regulatory hypomethylated regions. To investigate the possible effect of these regions on gene expression, a transcriptomics analysis was performed with mRNA isolated from the leukocyte subsets.