Monitoring Homology Search during DNA Double-Strand Break Repair in vivo

ABSTRACT: Homologous recombination (HR) is crucial for genetic exchange, accurate repair of DNA double-strand breaks and pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly understood. Here, we use time-resolved chromatin immunoprecipitations of repair proteins to monitor homology search in vivo. We found that homology search proceeds by a probing mechanism, which commences around the break and samples preferentially on the broken chromosome. However, elements thought to instruct chromosome loops mediate homology search shortcuts, and centromeres, which cluster within the nucleus, may facilitate homology search on other chromosomes. Our study thus reveale

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jörg Renkawitz 

PROVIDER: E-GEOD-44844 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress