Dynamics of yeast histone H2A and H2B phosphorylation in response to a double-strand break

ABSTRACT: In Saccharomyces cerevisiae, a single double-strand break (DSB) triggers extensive phosphorylation of histone H2A (known as gammaH2AX) over 50 kb on either side of the DSB. This modification is carried out by either of yeastM-^Rs checkpoint kinases, the ATM homolog, Tel1, or the ATR homolog, Mec1. In G1-arrested cells, where there is very little 5M-^R to 3M-^R processing of DSB ends, only Tel1 promotes this modification. We have recently described a second modification gammaH2B - the phosphorylation of the C terminal T129 locus of histone H2B which is also carried out by both Mec1 and Tel1 kinases. To understand in detail how gamma-H2AX and gamma-H2B spread along the chromosome from a DSB we have undertaken a high-density analysis of their occupancy where there is a DSB on three different

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Gaelle Legube 

PROVIDER: E-MTAB-1409 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress