The transcriptional response of Cryptococcus neoformans to ingestion by Acanthamoeba castellanii and murine macrophages

ABSTRACT: Virulence of Cryptococcus neoformans for mammals was proposed to emerge from evolutionary pressures on its natural environment by protozoan predators, which selected for strategies that allow survival within macrophages. In fact, Acanthamoeba castellanii ingests yeast cells, which then replicate intracellularly. In addition, most fungal factors needed to establish infection in the mammalian host are also important for survival within the amoeba. To better understand the origin of C. neoformans virulence, we compared the transcriptional profile of yeast cells internalized by amoebae and murine macrophages after 6 h of infection. Our results showed 656 and 293 genes whose expression changed at least two-fold in response to the intracellular environments of amoebae and macrophages, respective

ORGANISM(S): Cryptococcus neoformans

SUBMITTER: Michael Heinz 

PROVIDER: E-GEOD-45027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress