Transcription profiling of human pulmonary artery endothelial cell culture treated with Chapel Hill Ultrafine particle

ABSTRACT: Epidemiology studies have linked exposure to pollutant particles to increased cardiovascular mortality and morbidity, however, the mechanism remains unknown. In this study, we hypothesized that the ultrafine fraction of ambient pollutant particles would cause endothelial cells dysfunction. We profiled gene expression of human pulmonary artery endothelial cells (HPAEC) exposed to ultrafine Chapel Hill particles (UFP) (100μg/ml) or vehicle for 4h with Affymetrix HG U133 Plus 2.0 chips (N = 4 each). Using an unpaired t-test (p <0.01, 5% false discovery rate) we found 426 unique genes to be differentially expressed with 320 upregulated genes and 106 downregulated genes. Among these genes, we noted upregulation of genes related to coagulation-inflammation circuitry including tissue factor (

ORGANISM(S): Homo sapiens

SUBMITTER: Edward Karoly 

PROVIDER: E-GEOD-4567 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress