A stem cell model of diabetes due to glucokinase deficiency

ABSTRACT: Diabetes is a disorder characterized by loss of beta cell mass and/or beta cell function, leading to deficiency of insulin relative to metabolic need. To determine whether stem cell derived-beta cells faithfully reflect the phenotypes of a diabetic subject, we generated induced pluripotent stem cells from diabetic subjects (MODY2) with heterozygous loss-of-function of the gene encoding glucokinase (GCK). These stem cells differentiated into beta cells with an efficiency comparable to controls, and expressed markers of mature beta cells, urocotin-3 and zinc transporter 8 upon transplantation into mice. While insulin secretion in response to arginine or other secretagogues was identical to cells from healthy controls, GCK mutant beta cells required higher glucose levels to stimulate insulin

ORGANISM(S): Homo sapiens

SUBMITTER: Haiqing Hua 

PROVIDER: E-GEOD-45777 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress