Generation of functional human pancreatic beta cells in vitro

ABSTRACT: The generation of insulin-producing pancreatic cells from stem cells in vitro would provide an unprecedented cell source for drug discovery and cell transplantation therapy in diabetes. However, insulin-producing cells previously generated from human pluripotent stem cells (hPSC) lack many functional characteristics of bona fide β cells. Here we report a scalable differentiation protocol that can generate hundreds of millions of glucose-responsive β cells from hPSC in vitro. These stem cell derived cells (SC) express markers found in mature β cells, flux Ca2+ in response to glucose, package insulin into secretory granules and secrete quantities of insulin comparable to adult β cells in response to multiple sequential glucose challenges in vitro. Furthermore, these cells secrete human insul

ORGANISM(S): Homo sapiens

SUBMITTER: Jeffrey Millman 

PROVIDER: E-GEOD-61714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress