Serotyping of Streptococcus pneumoniae based on capsular genes polymorphisms

ABSTRACT: Diagnostic primer extension assay to serotype Streptococcus pneumoniae. Assay validation. Background: Monitoring of Streptococcus pneumoniae serotype epidemiology is essential since serotype replacement is a concern when introducing new polysaccharide-conjugate vaccines. To simplify S. pneumoniae serotyping, a novel PCR-based automated microarray assay was developed to assist in the tracking of the serotypes. Results: Autolysin (lytA), pneumolysin (ply) and eight genes located in the capsular operon (cps) were amplified using multiplex PCR. This step was followed by a tagged fluorescent primer extension step targeting serotype-specific polymorphisms. The tagged primers were then hybridized to a microarray. Results were exported to an expert system that transforms genetic typing data into

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Frederic Raymond 

PROVIDER: E-GEOD-45865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress