Mouse strain-specific responses to commensal gut bacteria at the mucosal gene expression level

ABSTRACT: The host genotype has been proposed to contribute to individually composed bacterial communities in the gut. To provide deeper insight into interactions between gut bacteria and their host, we associated germ-free C3H and C57BL/10 mice with intestinal bacteria from a C57BL/10 donor mouse. Analysis of microbiota similarity between the experimental animals with denaturing gradient gel electrophoresis (DGGE) 13 weeks after association revealed the development of a mouse strain specific microbiota. Gene expression in the colonic mucosa was analyzed with a microarray approach taking advantage of a modified Affymetrix mouse genome chip. We detected 202 genes whose expression differed significantly by a factor of < 2. Application of bioinformatics tools demonstrated that functional terms includin

ORGANISM(S): Mus musculus

SUBMITTER: Gunnar Loh 

PROVIDER: E-GEOD-45876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress