Transcription profiling of mouse uteri to evaluate the mechanism and physiological effects of estren

ABSTRACT: A proposed membrane-mediated mechanism of rapid non genomic response to estrogen has been the intense focus of recent research. Estren (Es), a synthetic steroid, is reported to act selectively through a rapid membrane-mediated pathway, rather than through the classical nuclear estrogen receptor (ER)-mediated pathway, to maintain bone density in ovaierectomized mice without uterotropic effects. To further evaluate the mechanism and physiological effects of Es we studied responses in adult ovariectomized mice. Experiment Overall Design: Mice were treated with sesame oil vehicle (Sigma), E2 (Steraloids; 1 µg/mouse) estren (300 µg/mouse), or dihydrotestosterone (DHT, Steraloids; 50 µg/mouse) and uteri were collected 2 or 24 hours after treatment and snap frozen in liquid nitrogen. Three o

ORGANISM(S): Mus musculus

SUBMITTER: NIEHS Microarray Group 

PROVIDER: E-GEOD-4615 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress