Transcription in Pronuclei and One- to Four-Cell Embryos Drives Early Development in a Nematode

ABSTRACT: A long-standing view of development is that transcription is silenced in the oocyte until early divisions in the embryo. The point at which major transcription is reactivated varies in different organisms, but is usually after the 2-cell stage. However, this model may not be universal. We used RNA-seq and exploited the protracted development of the parasitic nematode Ascaris suum, to provide a comprehensive time course of mRNA expression, degradation, and translation during early development. Surprisingly, we find that ~4,000 genes are transcribed prior to pronuclear fusion and in the 1-4 cell embryos. Intriguingly, we do not detect maternal contribution of many orthologs of maternal C. elegans mRNAs, but instead find these are newly transcribed in the A. suum zygote prior to pronuclear fu

ORGANISM(S): Ascaris suum

SUBMITTER: Jianbin Wang 

PROVIDER: E-GEOD-46277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress