Dataset Information


Identification of miRNAs regulated by TGF-b in human CD8+ T cells (small RNA-seq)

ABSTRACT: The goal of the current study was to identify miRNAs regulated by TGF-b in human CD8+ T cells and analyze the function of these miRNAs in shaping the immunomodulatory effect of TGF-b in these cells. We identified the miR-23a cluster to be upregulated and found that this cluster could target key molecules (IFN-g and LAMP1) involved in immune response by CD8+ T cells. CD8+ T cells were isolated and purified from healthy human peripheral blood of 5 donors and were activated using beads coupled to anti-CD2, anti-CD3 and anti-CD24. They were then treated with 5ng/ml TGF-b, with 1µM SD-208 or left untreated. RNA from these cells were then isolated and used for deep sequencing.

ORGANISM(S): Homo sapiens

SUBMITTER: Anoop Chandran P 

PROVIDER: E-GEOD-46322 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image


Cytokine secretion and degranulation represent key components of CD8(+) T-cell cytotoxicity. While transcriptional blockade of IFN-γ and inhibition of degranulation by TGF-β are well established, we wondered whether TGF-β could also induce immune-regulatory miRNAs in human CD8(+) T cells. We used miRNA microarrays and high-throughput sequencing in combination with qRT-PCR and found that TGF-β promotes expression of the miR-23a cluster in human CD8(+) T cells. Likewise, TGF-β up-regulated express  ...[more]

Similar Datasets

2013-05-28 | E-GEOD-45499 | biostudies-arrayexpress
2012-03-31 | E-GEOD-36971 | biostudies-arrayexpress
2014-03-07 | E-GEOD-39112 | biostudies-arrayexpress
2013-10-01 | E-GEOD-44802 | biostudies-arrayexpress
2014-08-01 | E-GEOD-46324 | biostudies-arrayexpress
2014-06-01 | E-GEOD-53451 | biostudies-arrayexpress
2016-05-15 | E-GEOD-57687 | biostudies-arrayexpress
2014-04-10 | E-GEOD-56614 | biostudies-arrayexpress
2013-08-27 | E-GEOD-50179 | biostudies-arrayexpress
2017-10-18 | E-GEOD-105052 | biostudies-arrayexpress