JMJD1C knockdown (H9_CT2_JMJD1C-KD_Microarray_JW)

ABSTRACT: It has been recently reported that the pluripotency factor OCT4, the early neural inducing factor NR2F2, and the pluripotency-associated miRNA miR-302 are linked in a regulatory circuitry that critically regulate both pluripotency and neural differentiation of human embryonic stem cells (hESCs). We show here that JMJD1C, a H3K9 demethylase expressed in undifferentiated hESCs, plays a key role in the regulatory circuitry. hESCs with JMJD1C knockdown (KD) retain the state of self-renewal and pluripotency, but express lower miR-302c than control hESCs. JMJD1C directly binds to the miR-302 promoter in hESCs and reduces H3K9 methylation on the promoter. Upon withdrawal of bFGF (an inhibitor of neural initiation) from a defined culture medium, the KD, but not control, hESCs differentiate into ne

ORGANISM(S): Homo sapiens

SUBMITTER: Ren-he Xu 

PROVIDER: E-GEOD-46485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress