The permanent reduction of TIA proteins molds expression transcriptome in HeLa cells

ABSTRACT: This study provides, for the first time, TIA1 and TIAR linked-transcriptomic analysis by using RNA-Seq next-generation sequencing technology. Illumina RNA-Seq was used to survey transcriptome profiles from permanent TIA1- and TIAR-(shRNA-mediated) deficient HeLa cells. Analysis of the transcriptomes with the Cufflinks tool revealed that differentially expressed genes, isoforms produced by alternative splicing and/or promoter usage as well as microRNAs generated a great transcriptomic heterogeneity which might reflect the complexity linked to these cell phenotypes. The data of differential expression were validated by using genome-wide microarrays and QPCR analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes term enrichment analysis revealed over-representation of genes assoc

ORGANISM(S): Homo sapiens

SUBMITTER: Juan Oliveros 

PROVIDER: E-GEOD-46516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress