Vascularised and functional human liver from an iPSC-derived organ bud transplant

ABSTRACT: Critical shortage of donor organs for treating end-stage organ failure highlights the urgent need for generating organs from induced pluripotent stem cells (hiPSCs). Despite many reports describing functional cell differentiation, no studies have succeeded in generating a three-dimensional vascularised organ such as liver. Here, we show the generation of vascularised and functional human liver from hiPSCs by transplantation of liver buds created in vitro (hiPSC-LBs). Specified hepatic cells self-organised into three-dimensional hiPSC-LBs by recapitulating organogenetic interactions between endothelial and mesenchymal cells. Immunostaining and gene expression analyses revealed resemblance between in vitro grown hiPSC-LBs and in vivo liver buds. Human vasculatures in hiPSC-LB transplants bec

ORGANISM(S): Mus musculus

SUBMITTER: Yasuharu Ueno 

PROVIDER: E-GEOD-46631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress