Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) have potential for personalized and regenerative medicine. While most methods of utilizing these cells have focused on deriving homogenous populations of specialized cells, there has been modest success in producing hiPSC-derived organotypic tissues or organoids. Here we present a novel approach for generating and then co-differentiating hiPSC-derived progenitors. With a genetically engineered pulse of GATA6 expression, we initiate rapid emergence of all three germ layers as a complex function of GATA6 expression levels and tissue context. Within two weeks we obtain a complex tissue that recapitulates early developmental processes and exhibits a liver bud-like phenotype, including haematopoietic and stromal cells, as well as a neuronal niche. O

ORGANISM(S): Homo sapiens

SUBMITTER: Ron Weiss 

PROVIDER: E-GEOD-74662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress