Comprehensive gene expression profile of extrahepatic bile ducts in mice with experimental biliary atresia

ABSTRACT: Newborn Balb/c mice were injected intraperitoneally with 1.5x10^6 fluorescent-forming units (ffu) of type- A Rhesus Rotavirus (RRV) or 0.9% normal saline (NS; control) within 24 hours of birth to induce experimental model of biliary atresia. Extrahepatic bile ducts including gallbladder were microdissected en bloc at 3, 7 and 14 days after RRV or saline injections. GeneChipM-BM-. Mouse Gene 1.0 ST Array (Affymetrix, CA) were used to screen mRNAs whose expression was differently regulated after RRV challenge compared to normal saline controls. Gene expression profiling: Each experimental conditon contains 3 sets of samples. Each sample consists of two to six extrahepatic bile ducts pooled prior to isolation of total RNA to ensure generation of adequate mRNA to perform quantitative experime

ORGANISM(S): Mus musculus

SUBMITTER: Jorge Bezerra 

PROVIDER: E-GEOD-46967 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress