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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from wild type and Gata6-deficient tissue resident peritoneal macrophages

ABSTRACT: Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. We resolved a restricted transcriptional profile for the self-renewing population of peritoneal resident macrophages, which is expressed during homeostasis and inflammation and distinct from other MM-CM-^X. Prominent within this profile was the expression of Gata6. This study represents a characterisation of the role of Gata6 in peritoneal resident macrophage phenotype. We used microarrays to determine the patterns of gene expression in peritoneal resident MM-CM-^X in the absence of GATA-6 against wild type. Conditional 'floxed' Gata6 deficient sex-matched mice between 7 weeks old were compared against wild type

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Stone

PROVIDER: E-GEOD-47049 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription factor Gata6 links tissue macrophage phenotype and proliferative renewal.

Rosas Marcela M Davies Luke C LC Giles Peter J PJ Liao Chia-Te CT Kharfan Bashar B Stone Timothy C TC O'Donnell Valerie B VB Fraser Donald J DJ Jones Simon A SA Taylor Philip R PR

Science (New York, N.Y.) 20140424 6184

Tissue-resident macrophages are heterogeneous as a consequence of anatomical niche-specific functions. Many populations self-renew independently of bone marrow in the adult, but the molecular mechanisms of this are poorly understood. We determined a transcriptional profile for the major self-renewing population of peritoneal macrophages in mice. These cells specifically expressed the transcription factor Gata6. Selective deficiency of Gata6 in myeloid cells caused substantial alterations in the ...[more]

PMID: 24762537

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microRNA transcriptome data from wild type and Gata6-deficient tissue resident peritoneal macrophages

Project description:microRNA transcriptome data from wild type and Gata6-deficient tissue resident peritoneal macrophages. Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. Gata6 itself has been shown to be a target of multiple miR. However, microRNA transcriptome and its dependence on tissue-specific macrophage programming, such as effected by GATA6, has not been explored. We used microRNA sequencing to determine the patterns of microRNA expression in peritoneal resident macrophages at homeostasis in the absence of GATA-6 against wild type.

2023-06-30 | E-MTAB-13039 | biostudies-arrayexpress

Expression data from wild type and Gata6-deficient tissue resident peritoneal macrophages

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2014-05-09 | GSE47049 | GEO

GATA6 dependent gene regulation in peritoneal macrophages

Project description:Peritoneal macrophages from control and Mac-Gata6 KO (LysM-cre;Gata6-floxed) mice were determined for genome wide gene expression. Sorted peritoneal macrophages from control and Mac-Gata6 KO mice were performed for whole genome expression analysis by Illumina microarray

2014-05-02 | E-GEOD-56684 | biostudies-arrayexpress

GATA6 dependent gene regulation in peritoneal macrophages

Project description:Peritoneal macrophages from control and Mac-Gata6 KO (LysM-cre;Gata6-floxed) mice were determined for genome wide gene expression.

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Human serous cavity macrophages and dendritic cells possess counterparts in the mouse with a distinct distribution between species

Project description:In mouse peritoneal and other serous cavities, the transcription factor Gata6 drives the identity of the major cavity resident population of macrophages, with a smaller subset of cavity-resident macrophages dependent on the transcription factor Irf4. Here we showed that GATA6+ macrophages in the human peritoneum were rare, regardless of age. Instead, more human peritoneal macrophages aligned with mouse CD206+ LYVE1+ cavity macrophages that represent a differentiation stage just preceding expression of Gata6. Low abundance of CD206+ macrophages was retained in C57BL/6J mice fed a high-fat diet or in wild-captured mice, suggesting that differences between serous cavity-resident macrophages in humans and mice were not environmental. Irf4-dependent mouse serous cavity macrophages aligned closely with human CD1c+CD14+CD64+ peritoneal cells that, in turn, resembled human peritoneal CD1c+CD14-CD64- cDC2. Thus, major populations of serous cavity-resident mononuclear phagocytes in humans and mice shared common features but the proportions of different macrophage differentiation stages greatly differ between the two species and DC2-like cells were especially prominent in humans.

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RNA transcriptome data from C57BL/6 tissue resident peritoneal macrophages over expressing microRNA 708 or control

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Transcriptional profiles of macrophages in resolving inflammation

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2014-05-09 | E-GEOD-28621 | biostudies-arrayexpress

RNA-seq gene expression profiles of primary murine peritoneal mesothelial cells.

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Gene expression profile in peritoneal macrophage stimulated with B-DNA or CpG-B

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2012-05-19 | E-GEOD-37854 | biostudies-arrayexpress

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Project description:IRF3 is one of the most critical transcription factor in down stream of pattern recognition receptors (such as toll-like receptor and RIG-I-like receptor) signalling pathway. IRF3 is known to induce the expression of type I IFN gene upon virus infection. To furter examine the role of IRF3 in virus-induced gene expression, we preformed microarray analysis in IRF3-/- peritoneal macrophages infected with VSV, and found that IRF3 suppresses the expression of Il12b gene. Peritoneal macrophages from WT of IRF3-/- B6 mice were infected with VSV(1 M.O.I. ) for 6 hous, and then subjected to microarray analysis.

2012-05-19 | E-GEOD-37855 | biostudies-arrayexpress

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