Effects of KAT2B and WDR5 depletion on hepatocyte gene expression

ABSTRACT: During fasting, increases in circulating pancreatic glucagon maintain glucose balance by up-regulating hepatic gluconeogenesis. Triggering of the cAMP pathway stimulates the gluconeogenic program through the phosphorylation of CREB and via the de-phosphorylation of the CREB coactivator CRTC2. Hormonal and nutrient signals are also thought to modulate gluconeogenic genes by promoting epigenetic changes that facilitate assembly of the transcriptional machinery, although the nature of these modifications is unclear. Here we show that histone H3 acetylation at Lys 9 (H3K9Ac) is elevated over gluconeogenic genes during fasting and in diabetes, where it contributes to increases in hepatic glucose production. Following its dephosphorylation, CRTC2 promoted increases in H3K9Ac by mediating the rec

ORGANISM(S): Mus musculus

SUBMITTER: Kim Ravnskjaer 

PROVIDER: E-GEOD-47179 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress