Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comparative analysis of D. shibae wild-type vs. M-bM-^HM-^FctrA, M-bM-^HM-^FchpT and M-bM-^HM-^FcckA

ABSTRACT: Comparison of D. shibae wild-type with single gene knock-out strains of a phosphorelay conserved in Alphaproteobacteria (M-bM-^HM-^FctrA, M-bM-^HM-^FchpT and M-bM-^HM-^FcckA) Loop-design, two biological replicates of D. shibae wild-type, M-bM-^HM-^FctrA, M-bM-^HM-^FchpT and M-bM-^HM-^FcckA in mid-exponential phase (OD 0.4) and stationary phase (6h after culture reached maximum OD)

ORGANISM(S): Dinoroseobacter shibae DFL 12

SUBMITTER: Juergen Tomasch

PROVIDER: E-GEOD-47451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Project description:Aerobic anoxygenic phototrophic bacteria (AAPs) of the Roseobacter group are abundant in the photic zone of the marine environment. Dinoroseobacter shibae, a typical representative, converts light into additional energy that enhances its survival under nutrient-depletion. AAPs produce cytotoxic singlet oxygenunder light exposition, but D. shibae developed mechanisms to counteract the lethal effects of illumination. Recent studies documented a pivotal role of extrachromosomal elements (ECRs) for the ecological fitness of roseobacters, and here we investigated their significance for the adaptation to specific environmental stress. D. shibae possessing five ECRs, i.e. three chromids and two plasmids, was starved for four weeks in the dark and light/dark cycles and the survival strategy was evaluated using transcriptomics. Few genes on the chromosome showed differential expression between non-starved and starved cells, in particular those with a role in oxidative stress response and photosynthesis. Extrachromosomal genes in contrast showed a systematic loss of transcriptional activity, especially in the dark starved cells. The observed silencing of gene expression was not due to plasmid loss, because all five ECRs were stably maintained. Exceptionally, the smallest 72-kb replicon was the least downregulated, and one region with genes for singlet oxygen stress response was even strongly activated under light/dark cycle. A ?72-kb curing mutant completely lost the ability to benefit from AAP under starvation, and we could thus document the essential role of the 72-kb chromid to light-stress adaptation. . Our data moreover suggest that the four ECRs of D. shibae without a vital function are transcriptionally silenced under starvation. Loop-design, two biological replicates of D. shibae DFL12T cultivated in artificial salt water medium with succinate as carbon source and 12/12 light-dark cycles. After reaching stationary phase the cells were starved for 4 weeks in the dark or under light-dark cycle.