Broad, ectopic expression of the sperm protein, PLCZ1 induces parthenogenesis and ovarian tumours in mice

ABSTRACT: Mammalian metaphase II (mII) exit and embryogenesis are induced at fertilization by a signal thought to come from the sperm protein, phospholipase C-zeta (PLCZ1). Meiotic progression can also be triggered without sperm, as in parthenogenesis, although the classic mouse in vivo parthenogenetic model, LT/Sv, fails in meiosis I due to an unknown molecular etiology. We here dissect PLCZ1 specificity and function in vivo and address its ability to interfere with maternal meiotic exit. Wild-type mouse Plcz1 expression was restricted to post-pubertal testes, and the brains of both sexes, with region-specifying elements mapping to a 4.1 kb Plcz1 promoter fragment. When broad ectopic PLCZ1 expression was forced in independent transgenic lines, they initially appeared healthy. Their oocytes und

ORGANISM(S): Mus musculus

SUBMITTER: Anthony Perry 

PROVIDER: E-GEOD-4822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress