Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer model (expression)

ABSTRACT: The cellular heterogeneity of one patient derived orthotopic breast cancer xenograft model (PDBCX) was investigated using flow cytometry , combined with assessment of in vivo tumorigenicity and whole genome expression profiling. Epithelial cell adhesion molecule (EpCAM) was revealed as a highly specific cell surface marker of the human tumor cell population in both xenografts. Based on expression patterns observed in primary tumor tissue, SSEA-4 and CD24 were chosen as markers to further subdivide the luminal tumor cells into four subpopulations. FACS sorting was used to isolate four cell subpopulations. Results: In vivo tumorigenicity assay showed that SSEA-4+/CD24+ cells were non-tumorigenic, while the three other subpopulations were tumorigenic. Tumors resulting from the SSEA-4+/CD2

ORGANISM(S): Homo sapiens

SUBMITTER: Nirma Skrbo 

PROVIDER: E-GEOD-48384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress