Evaluation of three different fluorescent dyes for cDNA labeling in microarrays

ABSTRACT: The simultaneous measurement of gene expression for thousands of genes in a single analysis by the microarray technology allows researchers to describe transcriptomes in various samples of interest. Problems with variation in data quality derived from microarray experiments are well known and might result from poor RNA quality, background problems, or sub optimal signal strength. To assess variation due to the fluorescent dye chosen, three different dye pairs were tested for labelling of cDNA in gene expression analysis experiments on a porcine immune focused oligonucleotide microarray (POM3). This in-house oligonucleotide microarray allowed a direct comparison of background fluorescence, Median signal intensities, numbers of spots detected, and resistance to photobleaching between differe

ORGANISM(S): Sus scrofa

SUBMITTER: Shila Mortensen 

PROVIDER: E-GEOD-4855 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress