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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The affect of specific ablation of Runx3 from Esam splenic dendritic cells

ABSTRACT: Esam/CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes Esam dendritic cells were freshly sorted from macs enriched splenic DCs taken from 6 weeks old mice. Four samples from four mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 conditional KO. Mice lacking Runx3 specifically in the DC compartment were produced by crossing Runx3fl/fl mice onto CD11c-Cre mice. This mating scheme generated Runx3fl/fl/CD11c:Cre (CD11c-DC-Runx3Δ) mice.

ORGANISM(S): Mus musculus

SUBMITTER: Yoram Groner

PROVIDER: E-GEOD-48590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional reprogramming of CD11b+Esam(hi) dendritic cell identity and function by loss of Runx3.

Dicken Joseph J Mildner Alexander A Leshkowitz Dena D Touw Ivo P IP Hantisteanu Shay S Jung Steffen S Groner Yoram Y

PloS one 20131015 10

Classical dendritic cells (cDC) are specialized antigen-presenting cells mediating immunity and tolerance. cDC cell-lineage decisions are largely controlled by transcriptional factor regulatory cascades. Using an in vivo cell-specific targeting of Runx3 at various stages of DC lineage development we show that Runx3 is required for cell-identity, homeostasis and function of splenic Esam(hi) DC. Ablation of Runx3 in DC progenitors led to a substantial decrease in splenic CD4(+)/CD11b(+) DC. Combin ...[more]

PMID: 24204843

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Runx3 function in CD4+ splenic dendritic cells

Project description:CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes CD4+ dendritic cells were sorted from freshly isolated macs enriched splenic DCs taken from 6 weeks old mice. Six samples from six mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 KO.

2014-06-22 | E-GEOD-48589 | biostudies-arrayexpress

Expression data in splenic DC subsets in wild type and Xbp1 deficient mice

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2014-01-01 | E-GEOD-52857 | biostudies-arrayexpress

Microarray analysis of WT ESAM+ and ESAM- cDC subsets

Project description:Defense against attaching and effacing (A/E) bacteria requires the sequential generation of IL-23 and IL-22 to induce protective mucosal responses. While the critical source of IL-22 has been identified as CD4+ and Nkp46+ innate lymphoid cells (ILCs), the precise source of IL-23 is unclear. Here, we use genetic techniques to deplete specific classical dendritic cell (cDC) subsets and analyze immunity to the A/E pathogen Citrobacter rodentium. We find that Zbtb46+ cDCs, and specifically Notch2-dependent intestinal CD11b+ cDCs, but not Batf3-dependent CD103+ cDCs, are required for IL-23 production and immunity against C. rodentium. Notch2 controls cDC differentiation at a terminal step mediated by lymphotoxin signaling. Importantly, these results provide the first demonstration of a non-redundant function of CD11b+ cDCs in vivo. Analysis of differentially expressed genes in ESAM+ and ESAM- CD11b+ and DEC205+ splenic classical DC subsets. Splenocytes were harvested from WT C57Bl/6 or WT Cx3cr1-gfp mice and cDC subsets sorted to >95% purity on the FACSAriaII.

2013-06-01 | E-GEOD-45679 | biostudies-arrayexpress

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2011-10-31 | E-GEOD-31551 | biostudies-arrayexpress

Runx3 regulated genes in splenic CD4+ dendritic cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-22 | E-GEOD-48591 | biostudies-arrayexpress

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2010-03-24 | E-GEOD-17322 | biostudies-arrayexpress

Gene expression profile in CD11c+ splenic dendritic cells and CD11c+CD103+ dendritic cells from mesenteric lymph nodes (MLN) of mice with DC-specific targeting of TGFBR2

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2012-07-25 | E-GEOD-39651 | biostudies-arrayexpress

Gene expression profiling of CD25High and CD25Low regulatory T cell populations from mice lacking autophagy in their dendritic cells and their littermate controls.

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Mouse lung CD103+ and CD11b-high dendritic cell (DC) subsets

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