Target specificity amongst canonical nuclear poly(A) polymerases in plants modulates organ growth and pathogen response

ABSTRACT: Polyadenylation of pre-mRNAs is critical for efficient nuclear export, stability and translation of the mature mRNAs, and thus for gene expression. The bulk of pre-mRNAs are processed by canonical nuclear poly(A) polymerase (PAPS). Both vertebrate and higher-plant genomes encode more than one isoform of this enzyme, and these are co-expressed in different tissues. However, in neither case is it known whether the isoforms fulfil different functions or polyadenylate distinct subsets of pre-mRNAs. Here we show that the three canonical nuclear PAPS isoforms in Arabidopsis are functionally specialized due to their evolutionarily divergent C-terminal domains. A strong loss-of-function mutation in PAPS1 causes a male gametophytic defect, while a weak allele leads to reduced leaf growth that resul

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michael Lenhard 

PROVIDER: E-GEOD-48821 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress