RNA-Seq of yeast FBY107, FBY106 strains to investigate pre-mRNA degradation pathway

ABSTRACT: General discard pathways eliminate unprocessed and irregular pre-mRNAs to control the quality of gene expression. In contrast to such general pre-mRNA decay, we describe here a nuclear pre-mRNA degradation pathway that controls the expression of select intron-containing genes. We show that the fission yeast nuclear poly(A)-binding protein, Pab2, and the nuclear exosome subunit, Rrp6, are the main factors involved in this polyadenylation-dependent pre-mRNA degradation pathway. Transcriptome analysis and intron swapping experiments revealed that inefficient splicing is important to dictate susceptibility to Pab2-dependent pre-mRNA decay. We also show that negative splicing regulation can promote the poor splicing efficiency required for this pre-mRNA decay pathway, and in doing so identify a

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Francois Bachand 

PROVIDER: E-MTAB-708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress