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DNA unmethylome profiling by covalent capture of CpG sites

ABSTRACT: Here we present an approach for the mapping of unmethylated DNA. Our technique is based on DNA methyltransferase-directed transfer of activated groups (mTAG) and covalent biotin tagging of unmodified CpG sites followed by affinity enrichment and interogation on tiling microarrays or next generation sequencing. mTAG labeled genomic DNA of one sample

ORGANISM(S): Homo sapiens

SUBMITTER: Viviane Labrie

PROVIDER: E-GEOD-48994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA unmethylome profiling by covalent capture of CpG sites.

Kriukienė Edita E Labrie Viviane V Labrie Viviane V Khare Tarang T Urbanavičiūtė Giedrė G Lapinaitė Audronė A Koncevičius Karolis K Li Daofeng D Wang Ting T Pai Shraddha S Ptak Carolyn C Gordevičius Juozas J Wang Sun-Chong SC Petronis Artūras A Klimašauskas Saulius S

Nature communications 20130101

Dynamic patterns of cytosine-5 methylation and successive hydroxylation are part of epigenetic regulation in eukaryotes, including humans, which contributes to normal phenotypic variation and disease risk. Here we present an approach for the mapping of unmodified regions of the genome, which we call the unmethylome. Our technique is based on DNA methyltransferase-directed transfer of activated groups and covalent biotin tagging of unmodified CpG sites followed by affinity enrichment and interrog ...[more]

PMID: 23877302

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