Impact of conditional autophagy knockout on cytotoxic CD8 T cells at day 7 and day 8 after influenza infection

ABSTRACT: To investigate the role of autophagy on cytotoxic CD8 T cells, we developed a new mouse that expresses an Atg5 gene with loxP sites up- and downstream of the exon 3, Cre-recombinase, T cell receptor specific for the OVA peptide SIINFEKL and the congenic marker Thy1.1. These cells were adoptively transferred into wild type mice followed by influenza-OVA infection and induction of Atg5 knockout at day 5 after infection. Tracking the kinetic of the cells show, that the Atg5 knockout cells do not expand as much as the control cells and they experience complete contraction within few days. No memory population is established. To investigate differences in Atg5 knockout cells versus control cells we sorted the donor cells at the peak of immune response and investigated their gene expression prof

ORGANISM(S): Mus musculus

SUBMITTER: Julian Lum 

PROVIDER: E-GEOD-49254 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress