Transcriptomic and Epigenetic Signatures of Mouse Cardiac Progenitor Cells (Methylation 3x720 CpG)

ABSTRACT: Adult cardioyocytes undergo remarkable dedifferentiation and cell cycle reprogramming/reentery when cultured in mitogen-rich medium, continuously, and give rise to cardiac progenitor cells (CPCs). Using microfluidic device for single-cell capture and whole-transcriptomic amplification, we analyze the whole-genome transcriptomic profile in adult mouse cardiomyocytes (Ctl) and in their derived CPCs, and validate the gene expression by single-cell PCR and PCR array. Using two platforms for DNA methylation profiling: NimbleGen DNA methylation array and CHARM array, the whole-genome DNA methylation profile in myocytes (Ctl) and CPCs were compared and their regulations in relationship to the transcriptomics profile were analyzed. The results demonstrated remarkable molecular reprogramming pertai

ORGANISM(S): Mus musculus

SUBMITTER: Yiqiang Zhang 

PROVIDER: E-GEOD-49678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress