Primate iPS cells as tools for evolutionary analyses

ABSTRACT: Induced pluripotent stem cells (iPSCs) are regarded as a central tool to understand human biology in health and disease. Similarly, iPSCs from closely related species should be a central tool to understand human evolution and to identify conserved and variable patterns of iPSC disease models. Here, we have generated human, gorilla, bonobo and cynomolgus monkey iPSCs. We show that these cells are well comparable in their differentiation potential and generally similar to human, cynomolgus and rhesus monkey embryonic stem cells (ESCs). RNA sequencing reveals that expression differences among clones, individuals and stem cell type are all of very similar magnitude within a species. In contrast, expression differences between closely related primate species are three times larger and most gene

ORGANISM(S): Macaca fascicularis

SUBMITTER: Martin Kircher 

PROVIDER: E-GEOD-50781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress