Gene expression timecourse in clock rescue strains

ABSTRACT: The goal of this experiment was to determine whether global circadian gene expression oscillation depends strictly on the presence of rpaA, even when the KaiABC post-translational oscillator is oscillating with a circadian period. Strains deleted for rpaA lack functional KaiABC post-translational oscillators because their reduced kaiBC expression level leads to a non-permissive Kai protein stoichiometry. We restored KaiABC post-translational oscillator function in a M-NM-^TrpaA M-NM-^TkaiBC strain by ectopic expression of kaiBC from the Ptrc promoter and used microarrays to measure the timecourse of gene expression globally. As a control, we used microarrays to measure the gene expression timecourse in a M-NM-^TkaiBC Ptrc::kaiBC strain, in which gene expression was expected to be rhythm

ORGANISM(S): Synechococcus elongatus PCC 7942

SUBMITTER: Joseph Markson 

PROVIDER: E-GEOD-50920 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress