Gene expression of the intermediate and outer interzone of murine embryos at 15.5 days of gestation

ABSTRACT: Nascent embryonic joints, interzones, contain a distinct cohort of progenitor cells responsible for the formation of the majority of articular tissues. However, to date the interzone has largely been studied using in situ analysis for candidate genes in the context of the embryo rather than using an unbiased genome wide expression analysis on isolated interzone cells, leaving significant controversy regarding the exact role of the intermediate and outer interzone layers in joint formation. Therefore, in this study, using laser capture microdissection (three biological replicates), we selectively harvested the intermediate and outer interzones of mouse embryos at gestational age 15.5 days, just prior to cavitation, when the differences between the layers should be most profound. Microarray

ORGANISM(S): Mus musculus

SUBMITTER: Florien Jenner 

PROVIDER: E-GEOD-51098 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress