Nanoscale Chromatin Profiling of Gastric Adenocarcinoma

ABSTRACT: To identify chromatin alterations in primary gastric adenocarcionomas, we performed nano-scale chromatin immunoprecipitation-sequencing (Nano-CHiPseq) of histone modifications in 5 gastric cancers and matched normal tissues, We identified hundreds of somatically-altered promoters (marked by H3K4me3) and enhancers (H3K4me1). The majority of cancer-associated promoters localized to genomic sites lacking previously-annotated transcription start sites (“cryptic promoters”), driving high expression of nearby genes implicated in gastrointestinal cancers, embryonic development, and tissue specification. Our findings demonstrate the feasibility of performing chromatin profiling on solid tumors where tissue is limiting, to identify in non-coding regions regulatory elements, transcriptional patterns

ORGANISM(S): Homo sapiens

SUBMITTER: Niantao Deng 

PROVIDER: E-GEOD-51776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress