Characterization of Gene Expression in liver cell

ABSTRACT: The Human Agilent 60-mer, oligonucleotide microarray consists of a total of probes covering 41,096 genes of human. Through a validation study, total RNA isolated from liver cells were used for comparison of gene expression profiles via microarray examination. A list of significantly differentially expressed genes was generated; 267 genes were up-regulated genes, and 65 genes were down-regulated genes. The Agilent microarray platform is based upon the hybridization of a single labeled sample (derived from RNA), followed by one-channel detection. The intensity of the hybridization signal is used to determine target concentration. In order to validate the technical quality of each probe in our arrays, we carried out 6 independent hybridizations on samples. To examine the gene expression pr

ORGANISM(S): Homo sapiens

SUBMITTER: Yang Bu 

PROVIDER: E-GEOD-51951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress