Microarray analysis of PC3 prostate tumor-initiating cells (PC3 holoclones)

ABSTRACT: BACKGROUND: Cancer stem-like cells are proposed to sustain solid tumors by virtue of their capacity for self-renewal and differentiation to cells that comprise the bulk of the tumor, and have been identified for a variety of cancers based on characteristic clonal morphologies and patterns of marker gene expression. METHODS: Single cell cloning and spheroid culture studies were used to identify a population of cancer stem-like cells in the androgen-independent human prostate cancer cell line PC3. RESULTS: We demonstrate that, under standard culture conditions, ~10% of PC3 cells form holoclones with cancer stem cell characteristics. These holoclones display high self-renewal capability in spheroid formation assays under low attachment and serum-free culture conditions, retain their holoclone

ORGANISM(S): Homo sapiens

SUBMITTER: David Waxman 

PROVIDER: E-GEOD-52161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress