Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

4C-seq indicate interactive sites with the sequence close from Meis2 promoter

ABSTRACT: We report the distribution of interactive sites with the sequence close from Meis2 promoter within the genome of mouse embryonic forebrain. We prepared the chromatin from 11 dpc embryonic forebrain and made 3C (chromosomal conformation capture) library. High-throughput sequencing applied for the 3C analysis revealed the distribution of modified interactive sites within developing forebrain. 4C-seq analysis of mouse 11 dpc embryonic forebrain with the sequence close from Meis2 promoter. Forebrain isolated and disected from 11 dpc embryos are fixed by 1% formaldehyde. After conventional 3C reaction, 3C library for highthroughput sequence is prepared by combination of adaptor ligation and nesting PCR reactions.

ORGANISM(S): Mus musculus

SUBMITTER: Takaho Endo 

PROVIDER: E-GEOD-52281 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Polycomb potentiates meis2 activation in midbrain by mediating interaction of the promoter with a tissue-specific enhancer.

Kondo Takashi T   Isono Kyoichi K   Kondo Kaori K   Endo Takaho A TA   Itohara Shigeyoshi S   Vidal Miguel M   Koseki Haruhiko H  

Developmental cell 20131226 1

Polycomb-group (PcG) proteins mediate repression of developmental regulators in a reversible manner, contributing to their spatiotemporally regulated expression. However, it is poorly understood how PcG-repressed genes are activated by developmental cues. Here, we used the mouse Meis2 gene as a model to identify a role of a tissue-specific enhancer in removing PcG from the promoter. Meis2 repression in early development depends on binding of RING1B, an essential E3 component of PcG, to its promo  ...[more]

Similar Datasets

Other 4C-seq indicate interactive sites with the sequence close from Meis2 promoter
2014-01-15 | GSE52281 | GEO
Genomics RING1B binding in the 11 dpc embryonic tissues
2014-01-14 | E-GEOD-48464 | biostudies-arrayexpress
Genomics Enhancer landscape of mouse developing forebrain at 11.5 dpc embryos [ChIP-seq]
2014-11-12 | GSE52280 | GEO
Genomics RING1B binding in the 11 dpc embryonic tissues
2014-01-14 | GSE48464 | GEO
Genomics,Multiomics Mus musculus
| PRJNA227331 | ENA
Genomics Enhancer landscape of mouse developing midbrain at 11.5 dpc embryos
2014-01-15 | E-GEOD-49652 | biostudies-arrayexpress
Genomics Enhancer landscape of mouse developing midbrain at 11.5 dpc embryos
2014-01-15 | GSE49652 | GEO
Genomics,Multiomics Mus musculus
| PRJNA210234 | ENA
Proteomics Stability of the neuronal fate determinant MEIS2 is controlled by calpain2
2024-02-08 | PXD036442 | Pride
Genomics Mus musculus
| PRJNA227332 | ENA