Indole Negatively Impacts Predation by Bdellovibrio bacteriovorus HD100 and Its Release from the Bdelloplast

ABSTRACT: Bdellovibrio bacteriovorus HD100 is a predatory bacterium which attacks a wide range of gram negative bacterial pathogens and is proposed to be a potential living antibiotic. In the current study, we evaluated the effects of indole, a bacterial signaling molecule commonly produced within the gut, on the predatory ability of B. bacteriovorus HD100. Indole significantly delayed predation on E. coli MG1655 and S. enterica KACC 11595 at physiological concentrations (0.25 to 1 mM) and completely inhibited predation when present at 2 mM. Microscopic analysis revealed that indole blocked the predator from attacking the prey. Furthermore, indole was not toxic to the predator but slowed down its motility. Microarray and RT-qPCR analyses confirmed this as the gene group showing the greatest down-reg

ORGANISM(S): Bdellovibrio bacteriovorus HD100

SUBMITTER: Robert Mitchell 

PROVIDER: E-GEOD-52455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress