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Quantitative proteome of whole predatory life cycle of periplasmic predatory bacterium B.bacteriovorus HD100 on E.coli K-12 MG1655 (LC-MSMS)

ABSTRACT: Bdellovibrio bacteriovorus is a Gram-negative predatory bacterium able to invade the periplasm of other Gram-negative bacteria. There, the predators feed on the prey cell contents before division and lysing the prey cell to escape and search for new prey. This project generates a highly resolved quantitative proteome for the entire predatory life cycle of B. bacteriovorus HD100 on Escherichia coli K-12 MG1655. The goal is to identify proteins with a specific pattern of abundance in different predatory life cycle phases, enabling a better understanding of the unique predatory mechanisms. This is achieved by quantifying and comparing proteins present at each predatory life cycle phase. Enhancing the understanding of B. bacteriovorus biology through this dataset can bolster its potential as a therapeutic tool against multidrug-resistant pathogens in the future.

INSTRUMENT(S):

ORGANISM(S): Bdellovibrio Bacteriovorus Hd100

SUBMITTER: Simona Huwiler

LAB HEAD: Simona Huwiler

PROVIDER: PXD056597 | Pride | 2025-10-16

REPOSITORIES: Pride

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			Action	DRS
	20230807_002_S545889_Pel10_pool19_Rep1_20230811171434.mgf	Mgf
	20230807_002_S545889_Pel10_pool19_Rep1_20230811171434.raw	Raw
	20230807_003_S545890_Pel10_pool20_Rep1_20230811191141.mgf	Mgf
	20230807_003_S545890_Pel10_pool20_Rep1_20230811191141.raw	Raw
	20230807_004_S545891_Pel10_pool21_Rep1_20230811210849.mgf	Mgf

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Bdellovibrio bacteriovorus attack phase and post-infection cells

Project description:Bdellovibrio bacteriovorus is a Gram-negative bacterium that is a pathogen of other Gram-negative bacteria, including many bacteria which are pathogens of humans, animals and plants. As such Bdellovibrio has potential as a biocontrol agent, or living antibiotic. B. bacteriovorus HD100 has a large genome and it is not yet known which of it encodes the molecular machinery and genetic control of predatory processes. We have tried to fill this knowledge-gap using mixtures of predator and prey mRNAs to monitor changes in Bdellovibrio gene expression at a timepoint of early-stage prey infection and prey killing in comparison to control cultures of predator and prey alone and also in comparison to Bdellovibrio growing axenically (in a prey-or host independent âHIâ manner) on artificial media containing peptone and tryptone. From this we have highlighted genes of the early predatosome with predicted roles in prey killing and digestion and have gained insights into possible regulatory mechanisms as Bdellovibrio enter and establish within the prey bdelloplast. Approximately seven percent of all Bdellovibrio genes were significantly up-regulated at 30 minutes of infection- but not in HI growth- implicating the role of these genes in prey digestion. Five percent were down-regulated significantly, implicating their role in free-swimming, attack-phase physiology. This study gives the first post- genomic insight into the predatory process and reveals some of the important genes that Bdellovibrio expresses inside the prey bacterium during the initial attack. Keywords: Transcriptional analysis 3 replicates of attack phase cells and 3 replicates of 30 minutes post-infection cells were analysed on individual arrays. Replicate 3 was normalized separately.

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Project description:Bdellovibrio bacteriovorus is a Gram-negative bacterium that is a pathogen of other Gram-negative bacteria, including many bacteria which are pathogens of humans, animals and plants. As such Bdellovibrio has potential as a biocontrol agent, or living antibiotic. B. bacteriovorus HD100 has a large genome and it is not yet known which of it encodes the molecular machinery and genetic control of predatory processes. We have tried to fill this knowledge-gap using mixtures of predator and prey mRNAs to monitor changes in Bdellovibrio gene expression at a timepoint of early-stage prey infection and prey killing in comparison to control cultures of predator and prey alone and also in comparison to Bdellovibrio growing axenically (in a prey-or host independent “HI” manner) on artificial media containing peptone and tryptone. From this we have highlighted genes of the early predatosome with predicted roles in prey killing and digestion and have gained insights into possible regulatory mechanisms as Bdellovibrio enter and establish within the prey bdelloplast. Approximately seven percent of all Bdellovibrio genes were significantly up-regulated at 30 minutes of infection- but not in HI growth- implicating the role of these genes in prey digestion. Five percent were down-regulated significantly, implicating their role in free-swimming, attack-phase physiology. This study gives the first post- genomic insight into the predatory process and reveals some of the important genes that Bdellovibrio expresses inside the prey bacterium during the initial attack. Keywords: Transcriptional analysis

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