EXPRSS: an Illumina based high-throughput expression-profiling method to reveal transcriptional dynamics - III

ABSTRACT: Background Next Generation Sequencing technologies have facilitated differential gene expression analysis through RNA-seq and Tag-seq methods. RNA-seq has biases associated with transcript lengths, lacks uniform coverage of regions in mRNA and requires 10M-bM-^@M-^S20 times more reads than a typical Tag-seq. Most existing Tag-seq methods either have biases or not high throughput due to use of restriction enzymes or enzymatic manipulation of 5M-bM-^@M-^Y ends of mRNA or use of RNA ligations. Results We have developed EXpression Profiling through Randomly Sheared cDNA tag Sequencing (EXPRSS) that employs acoustic waves to randomly shear cDNA and generate sequence tags at a relatively defined position (~150-200 bp) from the 3M-bM-^@M-2 end of each mRNA. Implementation of the method was verif

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ghanasyam Rallapalli 

PROVIDER: E-GEOD-52596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress