Benchmark study of different chromatin isolation methods using the E2Fa transcription factor

ABSTRACT: Genome-wide identification of transcription factor (TF) binding sites is pivotal to our understanding of gene expression regulation. Although much progress has been made in the determination of potential binding regions of proteins by chromatin immunoprecipitation (ChIP), this method has some inherent limitations regarding DNA enrichment efficiency and antibody necessity. Here, we report an alternative strategy for assaying in vivo TF-DNA binding in Arabidopsis thaliana cells by tandem chromatin affinity purification (TChAP). Evaluation of TChAP using the E2Fa TF and comparison with traditional ChIP and single chromatin affinity purification illustrates the suitability of TChAP and provides a resource for exploring the E2Fa transcriptional network. Integration with transcriptome, cis-regul

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ken Heyndrickx 

PROVIDER: E-GEOD-53422 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress