Genome-Wide Identification of the FRS12 Binding Sites by Tandem Chromatin Affinity Purification – Seq Analysis in Arabidopsis thaliana

ABSTRACT: To identify the genomic binding sites of FRS12, Tandem Chromatin Affinity Purification – Seq (TChAP-Seq) was performed on 7-d-old Pro35S:FRS12-HBH and Pro35S:NLS-GFP-HBH expressing Arabidopsis thaliana PSB-D cells. Cultures were transferred to long days (16:8) conditions two weeks before harvesting at night time (NT) at zeitgeber time (ZT) 20 (time of lights on usually defines zeitgeber time zero). Chromatin was isolated from formaldehyde-treated cell cultures following two affinity purification steps; first by IMAC using a Ni-NTA Superflow resin (Qiagen), then by a Biotin binding step using a Streptavidin Sepharose resin (GE Healthcare). Finally, protein-DNA bound fragments were decrosslinked, deproteinized and purified using QIAquick PCR Purification Kit (Qiagen). Pro35S:FRS12-HBH sam

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ken Heyndrickx 

PROVIDER: E-MTAB-3019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress