Global Transcriptome Analyses of Mammalian Terminal Erythroid Differentiation

ABSTRACT: Purpose:The purpose of this study is to create unbiased, stage-specific transcriptomes by RNA-seq analyses of pure populations of both murine and human erythroblasts at distinct developmental stages. Methods: Recently developed FACS-based methods (Chen et al, PNAS, Liu et al, Blood, Hu et al Blood) were employed to purify morphologically and functionally discrete populations of cells, each representing specific stages of terminal erythroid differentiation. RNA was prepared from these cells and subjected to RNA-seq analyses. Results: There were vast temporal changes in gene expression across the differentiation stages, with each stage exhibiting unique transcriptomes.Clustering and network analyses revealed that differing stage-specific patterns of expression across differentiation were tra

ORGANISM(S): Mus musculus

SUBMITTER: Vince Schulz 

PROVIDER: E-GEOD-53983 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress