Hit-and-run transcriptional control by bZIP1 mediates rapid nutrient signaling in Arabidopsis

ABSTRACT: To identify potential transient interactions between a TF and its targets, we developed an approach that can identify primary targets based either on TF-induced regulation or TF-binding, assayed in the same samples. Our studies focused on the TF bZIP1 (BASIC LEUCINE ZIPPER 1), a central integrator of cellular and metabolic signaling. To discern the mechanisms by which bZIP1 regulates a gene netowork in response to a nitrogen (N)-signal perceived in vivo, we perturbed both bZIP1 and the N-signal that it transduces in a cell-based transient expression system called TARGET (Transient Assay Reporting Genome-wide Effects of Transcription factors). To identify the genome-wide targets of bZIP1 in response to a N-signal, we transiently perturbed both the TF and the signal in the TARGET cell-based

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ying Li 

PROVIDER: E-GEOD-54049 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress