Transcription profiling time series of Pseudomonas aeruginosa wild type and lexA mutant response to treatment with ciprofloxacin

ABSTRACT: Pseudomonas aeruginosa infections can be virtually impossible to eradicate and the evolution of resistance during antibiotic therapy is a significant concern. In this study, we use DNA microarrays to characterize the global transcriptional response of P. aeruginosa to clinical-like doses of the antibiotic ciprofloxacin and also to determine the component that is regulated by LexA cleavage and the SOS response. We find that genes involved in virtually every facet of metabolism are down-regulated in response to ciprofloxacin. The LexA-controlled SOS regulon identified by microarray analysis includes only fifteen genes, but does include several genes that encode proteins involved in recombination and replication, including two inducible polymerases known to play a role in mutation and the

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Floyd Romesberg 

PROVIDER: E-GEOD-5443 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress