Transcriptome analysis of psoriasis in a large case-control sample: RNA-seq provides insights into disease mechanisms

ABSTRACT: To increase our understanding of psoriasis, we utilized RNA-seq to assay the transcriptomes of lesional psoriatic and normal skin. We sequenced polyadenylated RNA-derived cDNAs from 92 psoriatic and 82 normal punch biopsies, generating an average of ~38 million single-end 80-bp reads per sample. Comparison of 42 samples* examined by both RNA-seq and microarray [GSE13355] revealed marked differences in sensitivity, with transcripts identified only by RNA-seq having much lower expression than those also identified by microarray. RNA-seq identified many more differentially expressed transcripts enriched in immune system processes. Weighted gene co-expression network analysis (WGCNA) revealed multiple modules of coordinately expressed epidermal differentiation genes, overlapping significantly

ORGANISM(S): Homo sapiens

SUBMITTER: James Elder 

PROVIDER: E-GEOD-54456 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress