Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin

ABSTRACT: Epigenetic memory in induced pluripotent stem cells (iPSCs), with regards to their somatic cell type of origin, might lead to variations in their differentiation capacities. In this context, iPSCs from human CD34+ hematopoietic stem cells (HSCs) might be more suitable for hematopoietic differentiation than commonly used fibroblast-derived iPSCs. To investigate the influence of an epigenetic memory on the ex vivo expansion of iPSCs into erythroid cells, we compared iPSCs from human neural stem cells (NSCs) and human cord blood-derived CD34+ HSCs and evaluated their potential for differentiation into hematopoietic progenitor and mature red blood cells (RBCs). Although genome-wide DNA methylation profiling at all promoter regions demonstrates an epigenetic memory of iPSCs with regards to thei

ORGANISM(S): Homo sapiens

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-55109 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress