Origin-dependent neural cell identities in differentiated human iPS cells in vitro and after transplantation into the rodent brain

ABSTRACT: The differentiation capability of induced pluripotent stem cells (iPSCs) towards certain cell types for disease modelling and drug screening assays might be influenced by their somatic cell of origin. Here, we have compared the neural induction of human iPSCs generated from either fetal neural stem cells (fNSCs), dermal fibroblasts or CD34+ hematopoietic stem cells. Neural progenitor cells (NSCs) and neurons could be generated at similar efficiencies from all iPSCs. Whole genome transcriptome analysis and an expression analysis of neural genes in iPSC-derived NSCs revealed a separation of neuroectoderm- from mesoderm-derived cells. Furthermore, we found genes, which were similarly expressed between fNSCs and neuroectoderm- but not mesoderm-derived NSCs. Notably, these neural signatures wer

ORGANISM(S): Homo sapiens

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-55107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress